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. 2022 Apr 9;12:5999. doi: 10.1038/s41598-022-10057-7

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© The Author(s) 2022, corrected publication 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Antigenic reactivity of recombinant N and RBD proteins with human serum-specific SARS-CoV-2 antibody. In (A), CHO cells were transfected 72 h with plasmids expressing RBD-IgG2A (rN) and N-IgG2A (rRBD) proteins or empty vector plasmid (control). Aliquots of clarified cell culture supernatants (C3S) were analyzed by immunoblot assay using goat anti-mouse IgG heavy chain HRP antibody. The original full-length gel is presented in Supplementary Figure S1. In (B), pools of four COVID-19 donor serum samples (Table S2, serum donors n°16, 18, 19, and 20) (COVID-19 donor serum) and four negative donor serum samples (Table S3) (negative donor serum) at dilution 1:100 were tested for anti-N and anti-rRBD antibodies by indirect ELISA using rN and rRBD for protein-based antibody capture. The intensity values of serum samples were measured at O.D. 450 nm. The results are the mean (± SEM) of three replicates. Unpaired t tests between COVID-19 immune subject and infection-naïve individual were performed and statistically significant comparisons are shown as * p < 10^–4.