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. 2022 Apr 9;12:5999. doi: 10.1038/s41598-022-10057-7

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© The Author(s) 2022, corrected publication 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Antigenic reactivity of synthetic peptides with immune sera from a COVID-19 immune subject who received comirnaty vaccine. Serum samples from a COVID-19 patient were collected few weeks after recovery of SARS-CoV-2 infection (post-infection) and then two weeks after the injection of a single dose of comirnaty vaccine (vaccination). Vaccine administration was performed three months after COVID-19 recovery. In (A), serum samples at dilution 1:100 were assayed for the detection of antibodies against SARS-CoV-2 N and spike proteins by indirect ELISA using recombinant rN and rRBD proteins for antigen-based antibody capture. A pool of serum samples from infection-naïve individuals (Table S3) served as negative control serum. The intensity values of serum samples were measured at O.D. 450 nm and their immune reactivity was estimated as a fold increase of intensity values obtained with negative control serum. The results are the mean (± SEM) of three replicates. Statistically significant comparisons are shown as * p < 10^–4 (ns: non-statistically significant, p > 0.05). In (B), serum samples at dilution 1:50 were assayed for the detection of specific antibodies through peptide-based ELISA. The intensity values of serum samples were measured at O.D. 450 nm. The results are the mean (± SEM) of three replicates. Paired t tests on experimental points between post-infection and vaccination immune were performed and statistically significant comparisons are shown as * p < 10^–4. Differences between experimental points considered as non-statistically significant are not shown.