TABLE 1.

PCR primers for sequencing, cloning, site-directed mutagenesis, and amplification for transformation

Fragment	Primer	Nucleotide sequence (5′ to 3′)a	Position and direction
V149	rpoB-ri 1	CCCAACAGATTTAGAAGT	bp 54, sense
	RpoB-ri 1-B (BglII)	ATTA AGATCT CCCAACAGATTTAGAAGT	bp 54, sense
	rpoB-ri-F	GATCCCTTTGATGACAGAAC	bp 387, sense
	rpoB-ri-R	TACCATAACAGGCTCAGC	bp 916, antisense
	RpoB-ri-R-X (XhoI)	TTTAA CTCGAG TACCATAACAGGCTCAGC	bp 916, antisense
	V149X/Muta for	NNNAATCAACTCCACAGAAGCCb	bp 446, sense
	V149X/Muta rev	CACCACGCGCTCCACCC	bp 445, antisense
Cluster	RpoB5	AAATGATCACAAGCACCATC	bp 1530, sense
	rpoB-CL	ACCTTGCCATCCACAACC	bp 1839, antisense
	rpoB-CLF	ATGTGCCTGATTACATCACGAC	bp 1371, sense
	rpoB-CLR	TTGGCGCTGCATGTTAGTCC	bp 2106, antisense
R701	rpoB ecF	GGTAGCCGCATCGCTCATTC	bp 2028, sense
	rpoB ecR	TGCCTACAATGGGAGCGTC	bp 2146, antisense
	RpoB701F-B (BglII)	ATTA AGATCT CCTTTCCACTTTCACAAGAG	bp 1764, sense
	RpoB701F-X (XhoI)	TTTAA CTCGAG TTCCCTAACGCTAACTCG	bp 2423, antisense
	R710H-For	ACCAAGCGGTGCCGTTATTc	bp 2102, sense
	R701H-Rev	GCTGCATGTTAGTCCCCATTA	bp 2101, antisense

^aItalics indicate introduced restriction sites; underlining indicates introduced mutations. 

^bIntroduction of the completely randomized codon from bp 446 to 448, GTC to NNN. 

^cIntroduction of the mutation CGC to CAC into codon from bp 2101 to 2103.