Figure 1.

Map of neurofilament light species indicates that CSF NfL exists as multiple fragment species. Antibodies targeting various domains of NfL used for immunoprecipitation, coupled with mass spectrometry analysis, enabled identification of multiple NfL species in CSF. Light dotted lines represent potential fragments in NfL species identification, while dark solid lines represent identified fragment species. NfL species were identified using 23 different custom antibodies and data used to determine NfL species are shown in Supplementary Fig. 2.