REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal anti-MPS1	Millipore	Cat# 05-682, RRID:AB_11214479
Sheep anti-BUB1	Gift from S. Taylor (Taylor et al., 2001)	N/A
Rabbit monoclonal anti-BUBR1	Abcam	Cat#A b200062, RRID:AB_2910098
Mouse monoclonal anti-MAD1	Millipore	Cat# MABE867, RRID:AB_2910099
Mouse monoclonal anti-MAD2	Bethyl Laboratories	Cat# A300-300A, RRID:AB_309443
Rabbit polyclonal anti-CDC20	Bethyl Laboratories	Cat# A301-180A, RRID:AB_890559
Rabbit polyclonal anti-TPR	Novus Biologicals	Cat# NB100-2867, RRID:AB_10003336
Mouse monoclonal anti-ChTOG	Santa Cruz Biotechnology	Cat# sc-374394, RRID:AB_10987687
Rat monoclonal anti-CLASP1	(Pereira et al., 2009)	N/A
Rat monoclonal anti-CLASP2	(Pereira et al., 2009)	N/A
Rabbit polyclonal anti-CLIP-170	Gift from N. Galjart (Molines et al., 2020)	N/A
Rat monoclonal anti-EB1	Abcam	Cat# ab53358, RRID:AB_881313
Rat monoclonal anti-EB3	Gift from A. Akhmanova (Stepanova et al., 2003)	N/A
Mouse monoclonal anti-TTL	Proteintech	Cat# 66076-1-Ig, RRID:AB_11182924
Rabbit polyclonal anti-HURP (R140)	Gift from P. Meraldi (Silljé et al., 2006)	N/A
Rabbit polyclonal anti-Astrin C-terminal	Gift from D. Compton (Mack and Compton, 2001)	N/A
Rabbit polyclonal anti-HAUS6	Gift from R. Uehara (Uehara et al., 2009)	N/A
Rabbit polyclonal anti-KTNB1	Proteintech	Cat# 14969-1-AP, RRID:AB_10637861
Rabbit polyclonal anti-TPX2	Proteintech	Cat# 11741-1-AP, RRID:AB_2208895
Mouse monoclonal anti-HEC1	Abcam	Cat# ab3613, RRID:AB_303949
Rabbit polyclonal anti-Mis12	Gift from C. Sunkel (Feijão et al., 2013)	N/A
Rabbit polyclonal anti-SKA1	Gift from P. Meraldi (Gaitanos et al., 2009)	N/A
Rabbit polyclonal anti-SPINDLY	Gift from A. Desai (Gassmann et al., 2010)	N/A
Rabbit polyclonal anti-KNTC1	Gift from R. Gassmann (Gama et al., 2017)	N/A
Rabbit polyclonal anti-CENP-I	Gift from P. Meraldi (McClelland et al., 2007)	N/A
Mouse monoclonal anti-CENP-F	BD Biosciences	Cat# 610768, RRID:AB_398091
Rabbit polyclonal anti-CENP-C	Gift from W. Earnshaw (Saitoh et al., 1992)	N/A
Rabbit polyclonal anti-Aurora A	Novus Biologicals	NB100-267, RRID:AB_10002481
Rabbit polyclonal anti-Ninein	Gift from E. Nigg (Logarinho et al., 2012)	N/A
Mouse monoclonal anti-Aurora B	BD Biosciences	Cat# 611082, RRID:AB_2227708
Rabbit polyclonal anti-Survivin	Novus Biologicals	NB500-201, RRID:AB_10001517
Mouse monoclonal anti-INCENP	Santa Cruz Biotechnology	Cat# sc-376514, RRID:AB_11149761
Mouse monoclonal anti-NDE1	Abnova	Cat# H00054820-M01, RRID:AB_425994
Mouse monoclonal anti-Dynactin p150	BD Biosciences	Cat# 610473, RRID:AB_397845
Rabbit polyclonal anti-NuMA	Gift from D. Compton (Gaglio et al., 1995)	N/A
Rabbit polyclonal anti-DYNEIN	Thermo Fisher Scientific	Cat# PA5-49373, RRID:AB_2634827
Rabbit monoclonal anti-CENP-E (EPR4542(2))	Abcam	Cat# Ab133583, RRID:AB_2910100
Rabbit polyclonal anti-KIF20A (Mklp2)	Bethyl Laboratories	Cat# A300-879A-M, RRID:AB_2779522
Rabbit polyclonal anti-KIF2A	Gift from D. Compton (Ganem and Compton, 2004)	N/A
Rabbit polyclonal anti-KIF2C (MCAK)	Gift from D. Compton (Mack and Compton, 2001)	N/A
Rabbit polyclonal anti-KIF15	Cytoskeleton Inc.	Cat# AKIN13, RRID:AB_10708521
Rabbit polyclonal anti-KIF18A	Bethyl Laboratories	Cat# A301-079A, RRID:AB_873056
Rabbit polyclonal anti-KIF18B	Gift from J. Welburn (McHugh et al., 2018)	N/A
Rabbit polyclonal anti-KIFC1 (HSET)	Santa Cruz Biotechnology	Cat# sc-100947, RRID:AB_2132540
Rabbit polyclonal anti-KIF11	Sigma-Aldrich	Cat# HPA010568, RRID:AB_1848034
Rabbit polyclonal anti-KIF23	Proteintech	Cat# 28587-1-AP, RRID:AB_2881176
Rabbit polyclonal anti-KIF4A	Thermo Fisher Scientific	Cat# PA5-30492, RRID:AB_2547966
Mouse monoclonal anti-KIF22 (KID)	Gift from S. Geley	N/A
Mouse monoclonal anti-SGO1	Santa Cruz Biotechnology	Cat# sc-393993, RRID:AB_2910101
Rabbit polyclonal anti-CNDG2 (CAP-D2)	Novus Biological	Cat# NBP1-88202, RRID:AB_11014692
Rabbit polyclonal anti-SECURIN	Thermo Fisher Scientific	Cat# 700791, RRID:AB_2532345
Mouse monoclonal anti-SEPARASE	Santa Cruz Biotechnology	Cat# sc-390314, RRID:AB_2910102
Mouse monoclonal anti-CDK1	Santa Cruz Biotechnology	Cat# sc-54, RRID:AB_627224
Mouse monoclonal anti-CYCLIN-B1	Cell Signaling	Cat# 4135, RRID:AB_2233956
Rabbit polyclonal anti-PRC1	Santa Cruz Biotechnology	Cat# sc-8356, RRID:AB_2169665
Mouse monoclonal anti-PRC1	Santa Cruz Biotechnology	Cat# sc-56345, RRID:AB_630139
Mouse monoclonal anti-PLK1	Abcam	Cat# ab115763, RRID:AB_10902582
Rabbit polyclonal anti-STMN1	Proteintech	Cat# 11157-1-AP, RRID:AB_2197114
Rabbit polyclonal anti-VASH1	Proteintech	Cat# 12730-1-AP, RRID:AB_2288318
Mouse monoclonal anti-PICH	Millipore	Cat# 04-1540, RRID:AB_11210090
Mouse monoclonal anti-α-tubulin (clone B512)	Sigma Aldrich	Cat# T5168, RRID:AB_477579
Rabbit monoclonal anti-vinculin	Thermo Fisher Scientific	Cat# 700062, RRID:AB_2532280
Mouse monoclonal anti-GAPDH	Proteintech	Cat# 60004-1-Ig, RRID:AB_2107436
Human anti-CREST	Fitzgerald	Cat# 90C-CS1058, RRID:AB_1282595
Rat monoclonal anti-tyrosinated α-tubulin (clone YL1/2)	Bio-Rad	Cat# MCA77G, RRID:AB_325003
Mouse monoclonal anti-cMad2	Santa Cruz Biotechnology	Cat# sc-65492, RRID:AB_831526
Mouse monoclonal anti-γ-tubulin (Clone GTU-88)	Sigma-Aldrich	Cat# T6557, RRID:AB_477584
Mouse monoclonal anti-β-tubulin	Sigma-Aldrich	Cat#T5201
Rabbit monoclonal anti-β-tubulin	Abcam	Cat# T5201, RRID:AB_609915
Mouse monoclonal anti-α-tubulin (acetyl K40)	Abcam	Cat# ab24610, RRID:AB_448182
Rabbit anti-detyrosinated α-tubulin	Gift from M. Barisic (Liao et al., 2019)	N/A
Abberior donkey anti-human IgG STAR 580	Abberior Instruments	Cat# 2-0122-005-4, RRID:AB_2910104
Abberior goat anti-mouse IgG STAR 635p	Abberior Instruments	Cat# 2-0002-007-5, RRID:AB_2893232
Abberior goat anti-rat IgG STAR Red	Abberior Instruments	Cat# 2-0132-011-2, RRID:AB_2910106
Abberior Goat anti-rabbit IgG STAR 580	Abberior Instruments	Cat# 2-0012-005-8, RRID:AB_2910107
Abberior Goat anti-rabbit IgG STAR 635p	Abberior Instruments	Cat# 2-0012-007-2, RRID:AB_2893229
Goat anti-mouse Alexa Fluor® 488	Thermo Fisher Scientific	Cat# A-11029, RRID:AB_2534088
Goat anti-rabbit Alexa Fluor® 568	Thermo Fisher Scientific	Cat# A-11011, RRID:AB_143157
Goat anti-mouse Alexa Fluor® 568	Thermo Fisher Scientific	Cat# A-11031, RRID:AB_144696
Goat anti-rabbit Alexa Fluor® 488	Thermo Fisher Scientific	Cat# A-11008, RRID:AB_143165
Goat anti-human Alexa Fluor® 568	Thermo Fisher Scientific	Cat# A-21090, RRID:AB_2535746
Goat anti-mouse HRP	Jackson ImmunoResearch	Cat# 115-035-003, RRID:AB_10015289
Goat anti-rabbit HRP	Jackson ImmunoResearch	Cat# 111-005-003, RRID:AB_2337913
Donkey anti-sheep HRP	Jackson ImmunoResearch	Cat# 713-035-003, RRID:AB_2340709
Goat anti-rat HRP	Jackson ImmunoResearch	Cat# 112-035-003, RRID:AB_2338128
Chemicals, peptides, and recombinant proteins
MG132	Merck	Cat#474790-5MG
Nocodazole	Sigma-Aldrich	Cat#M1404-10MG
SiR-Tubulin	Spirochrome (Tebu-bio)	Cat#251SC002
SiR-DNA	Spirochrome (Tebu-bio)	Cat#251SC007
Centrinone	MedChem Express	Cat#HY-18682
Fibronectin	Sigma-Aldrich	Cat#F1141-1MG
DAPI (4′,6′-diamino-2-fenil-indol)	Sigma-Aldrich	Cat# D9542-1MG
Resazurin	Sigma-Aldrich	Cat#R7017
Janelia Fluor 646 (JF646)	Promega (VWR)	Cat#PROMGA1120
Deposited data
Additional videos and quantifications/charts for all analyzed siRNAs	This paper	http://indianmuntjac.i3s.up.pt
Experimental models: Cell lines
Immortalized female Indian muntjac fibroblasts	Gift from J. Shay (Zou et al., 2002)	N/A
Indian muntjac - H2B-GFP (female)	(Drpic et al., 2018)	N/A
Indian muntjac - EB3-Halotag/2xGFP-CENP-A	This paper	N/A
Indian muntjac - GFP-Centrin-1/2xGFP-CENP-A	This paper	N/A
Indian muntjac - GFP-α-Tubulin/mScarlet-CENP-A	This paper	N/A
Indian muntjac - PA-GFP-α-Tubulin/GFP-Centrin-1	This paper	N/A
Oligonucleotides
Table S1	Sigma-Aldrich	N/A
Recombinant DNA
pLVx-EB3-Halotag	This paper	N/A
pRRL-2xGFP-CENP-A	This paper	N/A
pLVx-Centrin-GFP	This paper	N/A
pLVX-mScarlet-CENP-A	This paper	N/A
pLVX-PA-GFP-α-Tubulin	This paper	N/A
pRRL-EGFP-α-Tubulin	(Ferreira et al., 2020)	N/A
pLVX-GFP-Centrin-1	Gift from Manuel Thery	Addgene #73331
Software and algorithms
Fiji/ImageJ	ImageJ	N/A
MATLAB 2018b; 2013b	The MathWorks	https://www.mathworks.com/products/matlab.html
Nikon Elements	Nikon Instruments Inc.	https://www.microscope.healthcare.nikon.com/products/software/nis-elements
Original MATLAB code	The MathWorks	https://doi.org/10.5281/zenodo.6326533