Figure 1.

Live cell imaging of reporter gene expression up to 27 days post-transduction. Arrows indicate the areas where the onset of reporter gene expression first appeared. Each retinal organoid was treated with 1E+10 genome copies, except for GRK1.GFP AAV8 Y733F, for which 5E+10 genome copies were used. Background fluorescence of live retinal organoids was unavoidable, but reporter expression was evident as higher intensity areas above this background. Further examples of retinal organoids treated per condition are shown in Supplementary Figure S2.