Fig. 2.

Bioactivity and morphological assay of the cryopreserved adipose tissue. A G3PDH assays in the CPA groups. All CPA groups were compared with fresh tissue. No significant difference was found between the 70% glycerol and fresh groups. B Images show HE-stained fat graft sections of 8 groups one month after fat grafting. All samples were cryopreserved at − 196 °C for 1 month. Necrosis was obvious in the blank group and the 80% glycerol group. A cyst is shown in the blank group. Scale bar: 250 µm. *p < 0.05, **p < 0.01, ***p < 0.001