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. 2022 Apr 8;13:147. doi: 10.1186/s13287-022-02824-0

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — EVs size, morphology and protein decoration analysis. a Cryo-EM images of EVs. Scale bars are 200 nm. b NTA size-profile of the isolated fractions. c Size profile and protein decoration analysis of the different fractions calculated by measuring Cryo-EM images. d Images of a non-decorated EV (I) and decorated EV (II) with their respective zoomed insets (right). Scale bars are 100 nm (left-sided images) and 20 nm (right-sided images)