FIGURE 1.

Water content, in percent weight of solid protein sample, as determined by TGA while heating at 4°C/min. Samples comprising 2 mg of GB1, CAHS D, or PvLEA4 in 650 μl 1.5 mM 4‐(2‐hydroxyethyl)‐1‐piperazineethanesulfonic acid, pH 6.5 were lyophilized for 24 hr, then exposed to 75% relative humidity at room temperature. Error bars represent the SD from three independent experiments. Molecular weight (MW), isoelectric point (pI), net charge calculated at pH 6.5, and FCRs are shown in the inset. CAHS D, cytosolic abundant heat‐soluble protein D; FCRs, fraction of charged residues; GB1, B1 domain of staphylococcal protein G; PvLEA4, LEA protein 4 from P. vanderplanki; TGA, thermogravimetric analysis