Figure 10.

Ca²⁺ infusion experiments confirm the Cl⁻/H⁺ exchanger dependency of the catecholamine accumulation process. A, Representative amperometric recording for WT, DMut, or DMut+ClC-3c (area highlighted in gray within the recording represents the first 40 s of analyses showed in C–G) stimulated by the pipette infusion of 3 μm free Ca²⁺. B, Representative single exocytotic events from amperometric recordings on WT+Scr (black), DMut (blue), DMut+ClC-3b (brown), or DMut+ClC-3c (gray). Spikes with values closer to the mean average amplitude, charge, and half for each condition were selected within the amperometric recordings. C–G, Properties of secretory amperometric events for WT+Scr (n = 23 cells, black circles, with 63.5 ± 9.6 events per cells), DMut (n = 20 cells, blue circles; with 33.9 ± 4.5 events per cell), DMut+ClC-3b (n = 18 cells, brown circles; with 31.2 ± 5.46 events per cells), or DMut+ClC-3c (n = 17 cells, gray circles; with 76.4 ± 7.7 events per cells), represented as data distribution. ***p < 0.001, **p < 0.01, *p < 0.05. ns, Not significant. Different conditions were compared with the WT+Scr or DMut using one-way ANOVA (Tukey's HSD post hoc test). Data were collected from four independent experiments per condition and are represented as mean ± SEM.