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. 2022 Apr 13;42(15):3080–3095. doi: 10.1523/JNEUROSCI.2439-21.2022

Figure 9.

Figure 9.

ClC-3c but not ClC-3b regulates catecholamine accumulation in chromaffin granules. A, Representative amperometric traces for WT+Scr, DMut, or DMut+ClC-3c condition. Secretion of catecholamine was triggered by the application of a high K+-extracellular solution (80 mm KCl, 60 s duration). B, Representative single exocytotic events from amperometric recordings on WT+Scr (black), Clcn3−/−+Scr, (red), DMut (blue), DMut+ClC-3b (brown), or DMut+ClC-3c (gray). CG, Properties of secretory amperometric events for WT+Scr (n = 24, black circles), Clcn3−/−+Scr, (n = 20, red circles), DMut (n = 23, blue circles), DMut+ClC-3b (n = 21, brown circles), or DMut+ClC-3c (n = 25, gray circles), represented as data distribution. ***p < 0.001, **p < 0.01, *p < 0.05. ns, Not significant. The different conditions were compared with WT+Scr or DMut using one-way ANOVA (Tukey's HSD post hoc test). Data were collected from seven independent experiments per condition and are represented as mean ± SEM. n denotes the number of analyzed cells.