Table 2.
Properties of individual fusion events stimulated by extracellular application of high [K+] solution in WT or Clcn3−/− chromaffin cells expressing scrambled/eGFP (WT+scr or Clcn3−/−+scr) or Clcn3−/− chromaffin cells expressing kdClC-5 (DMut) or kdClC-5+ClC-3b (DMut+ClC-3b) or kdClC-5+ClC-3c (DMut+ClC-3c)
| Foot parameters |
|||||||||
|---|---|---|---|---|---|---|---|---|---|
| Genotype (no. of cells) | No. events/stimulus | Charge (pC) | Amplitude (pA) | 50–90% Rise time (ms) | Half-width (ms) | % of events with foot | Foot amplitude (pA) | Foot duration (ms) | Foot charge (fC) |
| WT+Scr (n = 24) | 64.6 ± 7.0 | 0.206 ± 0.02 | 86.9 ± 6.2 | 0.34 ± 0.01 | 1.6 ± 0.1 | 41 ± 2.5 | 5.3 ± 0.2 | 8.1 ± 0.6 | 46.0 ± 4.2 |
| Clcn3-/-+Scr (n = 20) | 39.0 ± 5.6* | 0.158 ± 0.01* | 59.9 ± 6.3* | 0.37 ± 0.01 ns | 1.7 ± 0.1 ns | 43.7 ± 3.4 ns | 4.7 ± 0.3 ns | 8.6 ± 0.8 ns | 44.5 ± 4.7 ns |
| DMut (n = 23) | 40.0 ± 4.0* | 0.129 ± 0.009*** | 52.7 ± 4.5** | 0.35 ± 0.01 ns | 1.6 ± 0.1 ns | 43 ± 3.4 ns | 4.7 ± 0.3 ns | 7.4 ± 0.5 ns | 36.7 ± 3.8 ns |
| DMut+ClC-3b (n = 21) | 46.7 ± 6.1 ns/ns | 0.147 ± 0.011*/ns | 61.0 ± 7.4*/ns | 0.34 ± 0.01 ns/ns | 1.6 ± 0.1 ns/ns | 43.5 ± 3.9 ns/ns | 4.8 ± 0.4 ns/ns | 7.8 ± 0.6 ns/ns | 38.8 ± 4.6 ns/ns |
| DMut+ClC-3c (n = 25) | 68.3 ± 6.4 ns/†† | 0.208 ± 0.016 ns/††† | 92.3 ± 7.2 ns/††† | 0.34 ± 0.01 ns/ns | 1.6 ± 0.1 ns/ns | 47.7 ± 2.5 ns/ns | 5.3 ± 0.3 ns/ns | 9.2 ± 0.5 ns/ns | 46.6 ± 3.8 ns/ns |
No. of events/stimulus refers to the mean number of amperometric events obtained during the application of 80 mm potassium solution (60 s application).
***p < 0.001,
**p < 0.01,
*p < 0.05, ns; Not significant, versus WT+scr and
†††p < 0.001,
††p < 0.01,
†p < 0.05, ns, Not significant, versus DMut. One-way ANOVA (Tukey's HSD post hoc test). Data were collected from five to seven independent experiments per condition and are represented as mean ± SEM. Bold-italic ns represent the statistical comparison of the different rescues versus the DMut.