Table 3.
Properties of individual fusion events stimulated by intracellular perfusion of 3 μm free Ca2+ solution in WT chromaffin cells expressing scrambled/eGFP (WT+scr) or Clcn3−/− chromaffin cells expressing kdClC-5 (DMut) or kdClC-5+ClC-3b (DMut+ClC-3b) or kdClC-5+ClC-3c (DMut+ClC-3c)
| Genotype (no. of cells/total no. of events) | Event frequency (Hz) | Charge (pC) | Amplitude (pA) | 50–90% Rise time (ms) | Half-width (ms) | Foot parameters |
|||
|---|---|---|---|---|---|---|---|---|---|
| % of events with foot | Foot amplitude (pA) | Foot duration (ms) | Foot charge (fC) | ||||||
| WT+Scr (n = 23/1462) | 1.6 ± 0.24 | 0.241 ± 0.01 | 97.7 ± 5.0 | 0.33 ± 0.01 | 1.6 ± 0.1 | 25.9 ± 1.8 | 6.0 ± 0.4 | 6.3 ± 0.5 | 42.1 ± 4.4 |
| DMut (n = 20/801) | 0.84 ± 0.11* | 0.163 ± 0.009*** | 52.2 ± 4.2*** | 0.35 ± 0.01 ns | 1.9 ± 0.1* | 23.8 ± 2.2 ns | 4.7 ± 0.3 ns | 5.5 ± 0.3 ns | 28.0 ± 2.5* |
| DMut+ClC-3b (n = 18/717) | 0.8 ± 0.1*/ns | 0.139 ± 0.01***/ns | 64.8 ± 7.7**/ns | 0.32 ± 0.01 ns/ns | 1.4 ± 0.1 ns/††† | 23.9 ± 2.3 ns/ns | 4.7 ± 0.5 ns/ns | 5.6 ± 0.4 ns/ns | 31.6 ± 4.5 ns/ns |
| DMut+ClC-3c (n = 17/1375) | 1.9 ± 0.19 ns/††† | 0.224 ± 0.015 ns/† | 95.8 ± 6.1 ns/††† | 0.32 ± 0.01 ns/† | 1.5 ± 0.1 ns/†† | 29.5 ± 3.0 ns/ns | 5.6 ± 0.2 ns/ns | 4.8 ± 0.3 ns/ns | 30.0 ± 1.9 ns/ns |
No. of cells/total no. of events denotes the number of cell and the total number of events analyzed per condition. Event frequency was calculated over the first 40 s of amperometric recording.
***p < 0.001,
**p < 0.01,
*p < 0.05; ns, Not significant, versus WT+scr and
†††p < 0.001,
††p < 0.01,
†p < 0.05, ns, Not significant, versus DMut. One-way ANOVA (Tukey's HSD post hoc test). data were collected from four to five independent experiments per condition and are represented as mean ± SEM. Bold-italic ns represent the statistical comparison of the different rescues versus the DMut.