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. 2022 Apr 13;42(15):3066–3079. doi: 10.1523/JNEUROSCI.2065-21.2022

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Figure 4. — Contributions of GluN2-NMDARs to M-8324's impact on spontaneous spiking and E-S coupling in GABAergic neurons in vitro. A, Impact of TCN-201 (10 μm) on M-8324's effect on spontaneous spiking and E-S coupling. Spontaneous spiking, predrug, 1.01 ± 0.29 Hz; M-8324+TCN-201, 2.15 ± 0.71 Hz; RMP, −66.77 ± 2.12; N (cells) = 6. E-S coupling, predrug, 4.49 ± 0.20; M-8324+TCN-201, 6.34 ± 0.77; RMP, −67.09 ± 1.37 mV; N (cells) = 5. *p<0.05 (paired t test). B, Impact of Pip18 (1 μm) on M-8324's effect on spontaneous spiking and E-S coupling. Spontaneous spiking, predrug, 0.71 ± 0.12 Hz; M-8324+Pip18, 0.93 ± 0.17 Hz; RMP, −69.88 ± 2.97 mV; N (cells) = 8. E-S coupling, predrug, 4.63 ± 0.24; M-8324+Pip18, 5.21 ± 0.23; RMP, −65.62 ± 2.06 mV; N (cells) = 6. *p<0.05 (paired t test). C, Impact of NAB-14 (20 μm) on M-8324's effect on spontaneous spiking and E-S coupling. Spontaneous spiking, predrug, 0.91 ± 0.18 Hz; M-8324+NAB-14, 0.86 ± 0.20 Hz; RMP, −69.81 ± 1.56 mV; N (cells) = 8. E-S coupling, predrug, 7.32 ± 1.80; M-8324+NAB-14, 6.85 ± 1.41; RMP, −73.84 ± 2.38 mV; N (cells) = 5. *p<0.05 (paired t test). D, Impact of CIQ (10 μm, 5-10 min bath application) on spontaneous spiking and E-S coupling. Spontaneous spiking, predrug, 0.24 ± 0.03 Hz; CIQ, 0.60 ± 0.11 Hz; RMP, −73.12 ± 1.56 mV; N (cells) = 6. E-S coupling, predrug, 1.22 ± 0.70; CIQ, 2.26 ± 1.30; RMP, −69.52 ± 1.87 mV; N (cells) = 8. *p<0.05 (paired t test). E, Sample images showing the GluN2D protein expression level in the GluN2D-KD (E1). The population data (E2). Control, 1.34 ± 0.12; N (mice) = 4; GluN2D-KD, 0.95 ± 0.06; N (mice) = 4. F, Potentiation of M-8324 was absent in inhibitory neurons from GluN2D-NMDAR cKD mice. Spontaneous spiking, predrug, 0.69 ± 0.13 Hz; M-8324+NAB-14, 0.75 ± 0.19 Hz; RMP, −68.30 ± 0.94 mV; N (cells) = 7. E-S coupling, predrug, 4.84 ± 0.26; M-8324+NAB-14, 4.51 ± 0.14; RMP, −66.78 ± 1.9 mV; N (cells) = 7. Data are mean ± SEM.