Figure 10.

SCI altered activation of DH projection neuron subpopulations. Diagram of intraspinal viral injection experimental paradigm to selectively target ipsilateral DH (A). Experimental timeline (B). Representative transverse cervical spinal cord section of AAV1-flex-GFP virus injection 10 d post-tamoxifen administration; scale bar: 250 µm (C). Representative transverse cervical spinal cord images of FosTRAP2-labeled axons in the contralateral ALT, rostral to the injury site at 10 d post-tamoxifen induction in laminectomy (D) or SCI (E) animals. Representative coronal images of the LPB following laminectomy (F) or SCI (G); scale bar: 150 µm. LPB quantification of GFP⁺ axon profile counts (H) and total GFP⁺ axon length (I). Representative coronal images of the PAG following laminectomy (J) or SCI (K); scale bar 375 µm. Higher-magnification insets showing GFP⁺ axon fibers in the PAG following laminectomy (L) or contusion (M). PAG quantification of GFP⁺ axon profile counts (N) and total GFP⁺ axon length (O).