DH neuron activation changes induced by thermal stimulation. Representative DH images 35 d postsurgery following thermal stimulation of the ipsilateral forepaw in laminectomy: TdTomato (A), calretinin (B), and merge (C), and contusion: TdTomato (D), calretinin (E), and merge (F). Quantification of thermal stimulation induced activation of Lamina I cells (G), Laminae II+III cells (H), activated calretinin+ cells (I), and activated Laminae II+III Pax2+ inhibitory neurons (J). Representative ipsilateral DH images 35 d postsurgery following thermal stimulation in the ipsilateral forepaw in laminectomy: TdTomato (K), Pax2 (L), and merge (M), and contusion: TdTomato (N), Pax2 (O), and merge (P). White arrowheads indicate double-labeled cells. Scale bar: 100 µm.