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The Primer Sequences used for detecting Cytokine production by splenocytes
| Primer (Gene Location) | Sequence (5′–3′) |
|---|---|
| β-actin (NC_000071.7) | F: GGAGGGGGTTGAGGTGTT |
| R: GTGTGCACTTTTATTGGTCTCAA | |
| IL-2 (NC_000069.7) | F: CCCAAGCAGGCCACAGAATTGAAA |
| R: AGTCAAATCCAGAACATGCCGCAG | |
| IL-10 (NC_000067.7) | F: GGTTGCCAAGCCTTATCGGA |
| R: AATCGATGACAGCGCCTCAG | |
| IFN-γ (NC_000076.7) | F: CATCTTGGCTTTGCAGC |
| R: TTGTTGCTGATGGCCTG |
