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. 2022 Apr 9;79(5):230. doi: 10.1007/s00018-022-04269-3

Fig. 9.

Fig. 9

Lipid raft microdomain expression of Kvβ2.1 in the presence of PMA. HEK 293 cells were transfected with Kvβ2.1CFP. Isolation of lipid rafts was performed after 1 μM PMA incubation for 30 min at 37 °C. A Kvβ2.1CFP expression in lipid raft fractions in the absence of PMA. B Kvβ2.1CFP expression in the presence of PMA. C, D HEK 293 cells were cotransfected with Kvβ2.1CFP and Myc-PSD95 in the absence (C) or presence (D) of PMA. Immunoblot against CFP shows Kvβ2.1CFP. Immunoblot against Myc indicates Myc-PSD95. E Quantification of the Kvβ2.1 floatability, indicated by caveolin expression, relativized to the total amount of expression. **p < 0.01 vs. Kvβ2.1 in the absence of PMA (Student’s t test). Black bar, Kvβ2.1 alone; white bar, Kvβ2.1 in the presence of PMA; dark gray bar, Kvβ2.1 in the presence of PSD95 but in the absence of PMA; light gray bar, Kvβ2.1 in the presence of PSD95 and PMA. Values are men ± SE of 4 independent experiments. F Kvβ2.1 does not interact with PSD95. Cells were cotransfected with Kvβ2.1CFP and Myc-PSD95 in the absence (−) or presence (+) of PMA. Total lysates were coimmunoprecipitated against Kvβ2.1CFP (IP: CFP) and immunoblotted (IB) against CFP (Kvβ2.1) and myc (PSD95). SM, starting material; SN + : supernatant in the presence of antibody. IP + : Immunoprecipitation in the presence of the antibody