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. 2022 Apr 9;12:5994. doi: 10.1038/s41598-022-09787-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Representative micrographs at different magnifications (2000 × , 5000 × , and 10,000 ×) of the dentine surfaces after treatment with test and reference formulations, compared to the negative control (demineralized surface stored in PBS). Open tubules can be clearly seen that were closed in a variable percentage depending on the tested formulation. In general, mousses provided better obliteration (increased layer homogeneity) of the tubules than the toothpastes.