Fig. 3.

Hsa_circ_0001944 binds to miR-548b-3p. A The binding sites were predicted in Interactome. B miR-548b-3p was downregulated in CRC tissues. Paired t-test was applied. C The expression of hsa_circ_0001944 was negatively correlated with the expression of miR-548b-3p in CRC tissues (n = 133). Pearson correlation assay was applied. D miR-548b-3p was downregulated in CRC cells. E Inhibition of hsa_circ_0001944 increased the level of miR-548b-3p in CRC cells. Student t-test was applied. F, G RNA was affinity-isolated by biotinylated miR-548b-3p using biotinylated miRNA pull-down assay, and the hsa_circ_0001944 was quantified by qRT-PCR and normalized to input. Student t-test was applied. Error bars are mean ± standard error from at least three replicate samples. **P < 0.01, ***P < 0.001