Fig. 4.

5′-cap analysis. (a) SYBR-gold stained gel and immunoblot using cap-specific antibody (H20) indicating presence of cap in mutant (BY2739) and wild type S. cerevisiae (S288C ML). (b) SYBR-gold stained gel and Northern blot with 18S and 25S probes showing rRNA that has been treated with CAP-Clip or untreated. Decapping enzyme does not degrade RNA. (c) Gel and corresponding immunoblot using H20 antibody. (d) Quantitation of gel and immunoblot bands using ImageJ software. Direct comparison of untreated and Cap-Clip treated RNA is shown in each graph. Mean and standard deviation were calculated from three different experiments. All the gels and membranes were cropped to show relevant information. Full length gels and membranes with visible edges are shown in Fig. S7