Figure 1.

Ischemic preconditioning (IPC) does not result in cortical infarction but does protect against subsequent prolonged ischemia. A. Experimental paradigm describing the IPC stimulus and experimental end-points. B. Representative TTC-stained coronal brain tissue section images from four different animals that received a 15-minute tMCAO surgery 72 hours prior to perfusion/euthansia. Scale bar: 4 mm. C. Representative Cresyl violet-stained coronal brain tissue section images from two different animals that received a 15-minute tMCAO surgery 72 hours prior to perfusion/euthanasia. Scale bar: 4 mm. C’ is a 20x magnification view of the contralateral cortex within the MCA territory, and c” is the same view from the ipsilateral cortex. D. Representative TTC-stained coronal brain tissue section images of brains that received either a sham surgery or preconditioning surgery (15 minute tMCAO) 72 hours before a 60-minute tMCAO. E. Quantification of infarct volumes comparing brains that did (N = 6 animals) or did not (N = 4 animals) receive preconditioning 72 hours prior to a 60-minute tMCAO (t = 3.479, ***p = 0.0059; un-paired t-test).