FIGURE 2.

The PR1‐GFP (green fluorescent protein) trafficking is not dependent on endocytosis. (a) Insensitivity of the PR1‐GFP multivesicular body (MVB) compartment formation to co‐expressed endocytic inhibitor RFP‐HUB construct in Nicotiana benthamiana leaves. Bar = 10 μm. (b) Paracrine cell‐to‐cell spread of PR1‐GFP. Upper row: schematic of a situation in leaf epidermal cells indicative of the two‐construct expression in one cell (on the left) and cell‐to‐cell trafficking of one protein (PR1‐GFP) to the neighbouring cell (on the right). Middle: a schematic presentation of a vector encoding both PR1‐GFP and cytoplasmic construct SEC10‐mCherry as a reference. Lower row: cells that have only GFP signal (white arrow) and are neighbours to cells with both GFP and RFP signals. The penetrated signal was found to be of vesicular type (white arrows). Bar = 10 μm