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. 2022 Feb 4;23(5):664–678. doi: 10.1111/mpp.13187

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© 2022 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Subcellular localizations of red fluorescent protein (RFP)‐tagged PR1 variants. (a) Schematic representation of RFP‐tagged PR1 variants and their localization in infiltrated Nicotiana benthamiana leaves. Insets depict a magnified view of the signal in the dashed squares. A strong extracellular signal for FL and D11 variants, and endoplasmic reticulum and vacuolar localization of D50 and 4A was observed. In all cases a minor portion of the vesicular signal was detected as well. Bars = 10 μm. (b) The representative images showing corresponding localizations of FL, D50, 4A, and D11 variants tagged with RFP (upper row) and GFP (lower row). Bars = 100 μm