Table 1.
Cryo-EM data collection, processing, and refinement statistics
| Deposition ID | EMD-24815 | EMD-24814 | EMD-24816 | EMD-24832 |
|---|---|---|---|---|
| PDB: 7S21 | PDB: 7S20 | PDB: 7S2T | PDB: 7S4Q | |
| Data collection | ||||
| Magnification | 130,000 | 130,000 | 130,000 | 130,000 |
| Voltage (kV) | 300 | 300 | 300 | 300 |
| Total dose (e−/Å) | 66 | 66 | 46 | 46 |
| Frame rate | 4 frames/sec | 4 frames/sec | 5 frames/sec | 5 frames/sec |
| Defocus range (μm) | −0.6 to −2.6 | −0.6 to −2.6 | −0.6 to −2.6 | −0.6 to −2.6 |
| Pixel size | 1.06 | 1.06 | 0.537 | 0.537 |
| Data processing | ||||
| Symmetry | I1 | I1 | I1 | I1 |
| Initial particle number | 20706 | 20706 | 8139 | 10357 |
| Final particle number | 5183 | 9309 | 7961 | 8512 |
| Resolution (Å) | 3.4 | 3.4 | 3.5 | 3.1 |
| FSC threshold | 0.143 | 0.143 | 0.143 | 0.143 |
| Refinement | ||||
| Map sharpening B factor (Å2) | −90 | −90 | −90 | −90 |
| Model composition | ||||
| Non-hydrogen atoms | 2041 | 6364 | 6677 | 6659 |
| Protein residues | 265 | 825 | 862 | 862 |
| B factors (Å 2 ) | ||||
| Protein | 86.41 | 79.31 | 97.95 | 102.02 |
| R.m.s. deviations | ||||
| Bond length (Å) | 0.006 | 0.005 | 0.007 | 0.008 |
| Bond angles (°) | 0.765 | 0.729 | 0.903 | 0.928 |
| Validation | ||||
| Model-to-map fit CC protein | 0.84 | 0.80 | 0.80 | 0.81 |
| MolProbity score | 2.17 | 2.30 | 2.36 | 2.35 |
| Clashscore | 9.87 | 12.95 | 14.00 | 14.00 |
| Sidechain outliers (%) | 0.00 | 0.3 | 0.9 | 0.3 |
| Ramachandran | ||||
| Favored (%) | 85.44 | 83.88 | 81.88 | 82.59 |
| Allowed (%) | 14.56 | 16.12 | 18.00 | 17.41 |
| Disallowed (%) | 0.00 | 0.00 | 0.12 | 0.00 |