| Bacterial and virus strains |
| T7 express crystal competent E. coli
|
New England Biolabs |
Cat# C3022 |
| Chemicals, peptides, and recombinant proteins |
| LB broth base |
Invitrogen |
Cat# 12780-052 |
| Ampicillin |
Fisher Scientific |
Cat# BP1760-25 |
| Isopropyl-beta-D-thiogalactopyranoside |
MP Biomedicals |
Cat# 102101 |
| Tris base |
Fisher Scientific |
Cat# BP152-1 |
| Sodium Chloride |
KD Medical |
Cat# RGF-3270 |
| cOmplete EDTA-free protease inhibitor cocktail |
Roche |
Cat# 11873580001 |
| Triton-X100 |
MP Biomedicals |
Cat# 194854 |
| Tween-20 |
Sigma-Aldrich |
Cat# P-1379 |
| Lysozyme |
Chemical Dynamics Corporation |
Cat# 56-3660-00 |
| HisPur Ni-NTA resin |
ThermoFisher |
Cat# 88221 |
| Imidazole |
Sigma-Aldrich |
Cat# I3386-500G |
| HEPES |
Acros Organics |
Cat# 215001000 |
| 4–20% SDS-PAGE gels |
Invitrogen |
Cat# XV04200PK20 |
| O2-Ar gas (25–75%) |
Roberts Oxygen Company |
R0120508012 |
| Ethane gas |
Matheson Tri Gas |
5003838 |
| PEG 550 MME |
Hampton Research |
HR2-611 |
| PEG 20000 |
Hampton Research |
HR2–609 |
| Bicine |
Fluka |
Cat# 14871 |
| MPD |
Hampton Research |
HR2–627 |
| Calcium chloride |
Fisher Scientific |
Cat# C-79 |
| Iron chloride |
Sigma-Aldrich |
Cat# 372870 |
| Deposited data |
| EncA (T=1) cryo-EM map |
This paper |
EMD-24815 |
| EncA (T=3) cryo-EM map |
This paper |
EMD-24814 |
| EncA-EncB cryo-EM map |
This paper |
EMD-24816 |
| EncA-EncC cryo-EM map |
This paper |
EMD-24832 |
| EncA (T=1) structure |
This paper |
PDB: 7S21 |
| EncA (T=3) structure |
This paper |
PDB: 7S20 |
| EncA-EncB structure |
This paper |
PDB: 7S2T |
| EncA-EncC structure |
This paper |
PDB: 7S4Q |
| EncB crystal structure (space group P21) |
This paper |
PDB: 7S5C |
| EncB crystal structure (space group C2) |
This paper |
PDB: 7S5K |
| EncC crystal structure (space group C2) |
This paper |
PDB: 7S8T |
|
M. xanthus encapsulin protein EncA |
McHugh et al., 2014
|
PDB: 4PT2, EMD-5917 |
|
T. maritima FLP |
Sutter et al., 2008
|
PDB: 3DKT |
|
Q. thermotolerans IMEF |
Giessen et al., 2019
|
PDB: 6NJ8, EMD-9383 |
|
M. smegmatis encapsulin |
Tang et al., 2021
|
PDB: 7BOJ, EMD-30130 |
|
T. maritima encapsulin |
Xiong et al., 2020
|
PDB: 7K5W, EMD-22617 |
|
M. hassiacum encapsulin |
Loncar et al., 2020
|
PDB: 6I9G |
|
S. elongatus encapsulin |
Nichols et al., 2021
|
PDB: 6X8M, EMD-22094 |
| Rru_A0973 |
He et al., 2016
|
PDB: 5DA5 |
| PFC_05175 |
He et al., 2019
|
PDB: 5N5E |
| Hoch_3836 |
He et al., 2019
|
PDB: 5N5F |
| Recombinant DNA |
| EncA-pETDuet-1 |
This paper |
N/A |
| EncA-EncB-pETDuet-1 |
This paper |
N/A |
| EncA-EncC-pETDuet-1 |
This paper |
N/A |
| Software and algorithms |
| SerialEM |
Mastronarde, 2005
|
https://bio3d.colorado.edu/SerialEM/
|
| cisTEM |
Grant et al., 2018
|
https://cistem.org
|
| MolProbity |
Williams et al., 2018a
|
http://molprobity.biochem.duke.edu
|
| RELION-3.1 |
Scheres, 2012
|
https://www3.mrc-lmb.cam.ac.uk/relion/index.php/Main_Page
|
| MotionCor2 |
Zheng et al., 2017
|
https://emcore.ucsf.edu/ucsf-software
|
| UCSF Chimera |
Pettersen et al., 2004
|
https://www.cgl.ucsf.edu/chimera/
|
| Phenix |
Adams et al., 2010
|
https://phenix-online.org
|
| Coot |
Emsley et al., 2010
|
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
|
| XDS |
Kabsch, 2010
|
https://xds.mr.mpg.de
|
| XSCALE |
Kabsch, 2010
|
https://xds.mr.mpg.de/html_doc/xscale_program.html
|
| Phaser |
McCoy et al., 2007
|
https://www-structmed.cimr.cam.ac.uk/phaser_obsolete/
|
| UCSF ChimeraX |
Goddard et al., 2018
|
https://www.cgl.ucsf.edu/chimerax/
|
| PDBePISA |
Krissinel and Henrick, 2007
|
https://www.ebi.ac.uk/pdbe/pisa/
|
| Other |
| Beckman Coulter Avanti J30I refrigerated centrifuge |
Beckman Coulter |
https://www.beckman.com/centrifuges
|
|
|
|
| Leica EM GP Plunger |
Leica Microsystems |
https://www.leica-microsystems.com
|
| Plasma Cleaner Model 1020 |
Fischione |
https://www.fischione.com
|
| Quantifoil R1.2/1.3 300 mesh Cu grids coated with 2 nm ultrathin carbon |
Electron Microscopy Sciences, Protochips, Inc. |
Cat# Q310CR1.3–2nm |
