FIGURE 2.

Erastin increases the death of Schwann cells and the accumulation of ROS. (A) The expression of the molecular marker S‐100 in RSC 96 and pSCs (scale bar =100 μm). (B) The viability of RSC 96 and pSCs decreased after incubation with erastin for 24 h by CCK‐8 assay. (C) Cell death was confirmed by PI and Hoechst 33342 staining for 24 h (scale bar =200 μm). (D) The effect of erastin on intracellular ROS accumulation in RSC 96 and pSCs by immunofluorescence (Scale bar =200 μm). (E) Fluorescence intensity analysis of intracellular ROS accumulation in RSC 96 and pSCs. Data are presented as mean ± SD. T‐test and one‐way ANOVA were performed for statistical differences. All experiments were performed at least 3 times independently, **p < 0.01, ***p < 0.001, ****p < 0.0001 compared to the control group