FIGURE 3.

Erastin induces ferroptosis in RSC 96 and pSCs. (A, D) The expressions of GPX4 and PTGS2 proteins were induced by 0–2 μM erastin in RSC 96 and pSCs by Western blot. (B, E) Densitometric analysis of GPX4 and PTGS2 proteins relative expression in RSC 96 and pSCs. (C, F) The expression of GPX4 and PTGS2 induced by 1 μM erastin in RSC 96 and pSCs by immunofluorescence (scale bar =50 μm). (G) 1 μM Erastin decreased the GSH level and increased the MDA and Fe2+ level in RSC 96 and pSCs by ELISA. Data are presented as mean ±SD, and one‐way ANOVA and t‐test were performed for statistical differences. All experiments were performed at least 3 times independently, **p < 0.01, ***p < 0.001, ****p < 0.0001 compared to the control group