FIGURE 4.

NRF2/HO‐1 pathway is involved in erastin‐induced ferroptosis in RSC 96 and pSCs. (A, C) The expression of NRF2/HO‐1, and c‐Jun protein in RSC 96 and pSCs by Western blot. (B, D) Densitometric analysis of NRF2/HO‐1 and c‐Jun proteins relative expression in RSC 96 and pSCs. (E, F) Immunofluorescence showed that the expression of c‐Jun in RSC 96 and pSCs induced by 1 μM erastin (scale bar =50 μm). Data are presented as mean ± SD, and one‐way ANOVA was performed for statistical differences. All experiments were performed at least 3 times independently, *p < 0.05, **p < 0.01, compared to the control group