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Multiplex PCR assay performed for the detection of the MABC subspecies within a mixed sample. The mixed sample contained the extracted DNA of M. abscessus, M. bolletii, M. massiliense and other bacterial samples (M. chelonae, M. tuberculosis, M. avium, M. intracellulare, M. chimaera and E. coli) (controls) in the same tube. The PCR products sizes for the MABC subspecies are shown in the multiplex assays (Lane A–C).
