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. 2021 Jun 3;71(5):928–937. doi: 10.1136/gutjnl-2020-323219

Figure 3.

Figure 3

L cells and enterochromaffin (EC) cells coexpress GPR84 and FFAR4 in human proximal colon. (A) GPR84 is expressed on GLP-1/PYY containing L cells and 5-HT containing EC cells in human proximal colon mucosa. Expression of GPR84 was highest in EC cells, with a smaller population of L cells expressing this GPCR. n=5. (B) FFAR4 expression was observed on both GLP-1 and PYY containing L cells, although in a small subpopulation. EC cells, however, expressed FFAR4 in majority of 5-HT containing cells. n=5. (C) Top panel: FFAR4 and GPR84 are coexpressed on GLP-1 containing L cells, as shown in the merged image. Analysis of coexpression is shown in the graphs where cells were analysed for expressing GLP-1 alone, FFAR4 alone, GPR84 alone and when all three were found expressed on the same cell as shown by ‘merge’. n=5. Bottom panel: FFAR4 and GPR84 are coexpressed on 5-HT containing EC cells. Analysis of coexpression is shown in the graphs where cells were analysed for expressing 5-HT alone, FFAR4 alone, GPR84 alone and when all three were found expressed on the same cell as shown by ‘merge’. n=5.