Figure 3.

LmWT challenge leads to rapid accumulation of TRM cells in the skin of immunized or healed mice. Fifteen-week (15w) immunized and healed mice and non-immunized control mice were challenged in the flank skin with LmWT virulent parasite. Skin from the site of challenge was collected at 48 and 72 hours (48h and 72h) post-challenge. Tissue infiltration and TRM cells were analyzed by immunofluorescence and H&E staining. (A) Schematic plan of the experimental time points. (B) H&E staining of skin tissue at the site of challenge 48 h post-challenge. Black arrow indicates cellular infiltration at the challenge site. (C) Expression of CD3 (red), CD69 (blue), and CD103 (green) in the flank skin of non-immunized control, healed, and immunized mice, at the site of challenge, following 48 and 72 h post-challenge. Scale bar is 25 µm. Results are representative of one independent experiment, repeated 3 times, with 3 mice per group.