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. 2022 Mar 28;13:878049. doi: 10.3389/fmicb.2022.878049

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Copyright © 2022 Jeon and Cho.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Overexpression of prc or nlpI causes synthetic lethality with mepS and mepM mutations in minimal medium. (A) MG1655(attHKpWJ90, P_tac::empty), MG1655 (attHKpWJ186, P_tac::prc), HC611[P_ara::mepS, ΔmepM](attHKpWJ90), WJ283(P_ara::mepS, ΔmepM, ΔmepH;attHKpWJ90), HC611(attHKpWJ186) strains grown overnight in M9-arabinose medium were diluted in M9-glucose lacking casamino acids (CAA) and spotted on M9-glucose (no CAA) agar lacking or containing 100 μM IPTG for induction of prc expression. The plates were photographed after incubation for 40 h at 37°C. (B) MG1655(attHKpWJ90), MG1655 (attHKpWJ72, P_tac::nlpI), HC611(attHKpWJ90), WJ283(attHKpWJ90), HC611(attHKpWJ72) strains were grown, diluted, and spotted in the same way as in (A) except that 25 μM IPTG was used for induction of nlpI expression. The plates were photographed after incubation for 40 h at 30°C.