Figure 7.

Prc degrades MepH and this degradation is enhanced by NlpI. Time course experiment for MepH degradation in the presence or absence of Prc and NlpI. Four different reactions that contained the indicated proteins were prepared in PBS buffer and incubated at 37°C. Note that His6-SUMO-tagged MepH (H-SUMO-MepH) was used instead of MepH because untagged MepH was not soluble. H-SUMO-MepH is labeled as MepH in this figure for convenience. The concentrations of each protein added to the reactions were 5 μM (MepH), 1 μM (Prc), and 2 μM (NlpI). Aliquots were taken from each reaction at the indicated time points, mixed with Laemmli sample buffer, and boiled. Samples were separated on 12% SDS-PAGE gels and the gels were stained with Coomassie brilliant blue. Positions of molecular weight markers are indicated on the left and positions of the proteins used in the assay are indicated with arrows on the right.