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. 2022 Mar 28;13:821030. doi: 10.3389/fmicb.2022.821030

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Copyright © 2022 Nepel, Angel, Borer, Frey, MacDougall, McCulley, Risch, Schütz, Seabloom and Woebken.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Diazotrophic community composition per grassland site. (A) Taxonomic affiliation of OTUs summarized at the class level based on BLASTP analysis; (B) relative read abundances of phylogenetic nifH clusters based on classification and regression trees (CART) analysis. On the x-axis, every bar represents the average community per study site, sorted according to longitudinal coordinates, whereas the y-axis shows the relative read abundance of taxonomic classes (A) or phylogenetic nifH clusters (B). Shapes above the grassland site names denote the respective continent (circle: North America, square: Europe, diamond: Africa, triangle: Australia).