Extended Data Fig. 3 ∣. Describing phenotypes of DKO^T, KO^T and TKO^T CD8⁺ T cells.

Flow cytometry analysis of markers of activation and exhaustion (a) and BATF transcription factor expression (b) in splenic WT, DKO^T, KO^T and TKO^T CD8⁺ T cells. Data are representative of at least 5 individual 6-12 week old mice per genotype in at least two independent experiments. Flow cytometry analysis to Fig. 3e, f: intracellular cytokine staining of IFN-γ, TNF (c) and IL-2 (d) after PMA/ionomycin stimulation in CD8⁺ T cells of splenocytes from WT, DKO^T, KO^T and TKO^T mice for 4 h. Histogram analysis to Fig. 3g: Granzyme B expression in splenic WT, DKO^T, KO^T and TKO^T CD8⁺ T cells (e).