Fig. 6 ∣. Inhibition of Roquin-1 interaction with Regnase-1 breaks T cell quiescence.

a–h, CD4⁺ and CD8⁺ T cells from mice with L209Y and M199R mutations (10–12 weeks old) or from mice with E212K mutations (8 weeks old) in Roquin-1 were characterized. CD4⁺ effector/memory populations (a,b), CD4⁺ T cell proliferation (c,d), ability of CD4⁺ T cells to produce IFN-γ and IL-17 after PMA/ionomycin stimulation ex vivo (e,f) or CD8⁺ effector/memory populations (g,h) were compared. i–k, Frequencies of CD4⁺ effector/memory T cell populations (i), T_FH cells (j) and GC B cells (k) from spleens of 9–14-week-old WT, Rc3h1^M199R/fl;Cd4-Cre, Rc3h1^M199R/fl;Vav-Cre and Rc3h1^M199R/M199R (sanroque) mice were determined by flow cytometry. Contour plots are representative of n = 4 biological replicates analyzed in at least two independent experiments (a,c,e,g) or n = 2 biological replicates in two independent experiments (b,d,e,h). Data are presented as mean ± s.e.m. of WT, Rc3h1^M199R/M199R, n = 6; Rc3h1^M199R/fl, Cd4-Cre, Rc3h1^M199R/fl, Vav-Cre, n = 5 (i) or WT, Rc3h1^M199R/M199R, n = 8; Rc3h1^M199R/fl, Cd4-Cre, n = 6; Rc3h1^M199R/fl, Vav-Cre, n = 7 (j,k) analyzed mice in at least three independent experiments. Statistical significance was calculated by one-way ANOVA with Bonferroni post hoc test (j,k).