Extended Data Fig. 1 ∣. Roquin-1/2 and Regnase-1 maintain quiescence of T cells.

(a, b) Analysis of mixed bone marrow chimeric mice using either WT (CD45.2) and WT (CD45.1) or TKO^T (CD45.2) and WT (CD45.1) bone marrow cells injected into lethally irradiated CD45.1/2 recipient mice. Flow cytometry analysis of CD45.1 and CD45.2 cell populations (a) or T_reg cells (b) in splenocytes from recipient mice 9 weeks after reconstitution (WT CD45.1/WT CD45.2 recipients: n = 2, WT CD45.1/TKO CD45.2 recipients: n = 3, analyzed in one experiment). (c) H&E sections of lungs showing alveoli from WT, DKO^T, KO^T and TKO^T mice at the age of 6-8 weeks (Representative data of n ≥3 individual mice). (d, e) Analysis of CD45.2⁺ CD3⁺ T cells from Cre-ERt2 (WT), Rc3h1/2^fl/fl; Cre-ERt2 (iDKO), Zc3h12a^fl/fl; Cre-ERt2 (iKO) and Rc3h1/2^fl/fl; Zc3h12a^fl/fl; Cre-ERt2 (iTKO) mice that were adoptively transferred into WT CD45.1⁺ mice. Recipient mice were treated with tamoxifen by oral gavage to induce deletion of floxed alleles. On day 8 post transfer, T cells were analyzed for their ability to acquire an effector/memory phenotype (d) or to proliferate (e) within the host (n = 6 biological replicates).