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Gating methods to exclude debris, dead cells, and doublets. (A) Exclusion of debris/dead cells and gating of cells (red frame) to be included in subsequent steps of the analysis based on the forward scatter (FSC, related to size) and side scatter (SSC, related to granularity). (B) Gating of single cells (singlets) based on forward scatter width (FSC-W) and forward scatter height (FSC-H) and exclusion of cell clumps (doublets).
