TABLE 1.
SAXS‐derived structural parameters for p66L289Ktr
| Concentration (mg/ml) | Guinier analysis | Distance distribution analysis | Molecular weight a | ||||||
|---|---|---|---|---|---|---|---|---|---|
| I 0 | R g (Å) | I 0 | R g (Å) | D max (Å) | Porod volume b | Volume of correlation b | Size & Shape b | MoW2 c | |
| 1.0 | 0.029 | 31.72 ± 0.63 | 0.029 | 32.07 ± 0.43 | 107 | 67.3 | 62.6 | 79.2 | 71.5 |
| 2.0 | 0.058 | 31.95 ± 0.40 | 0.058 | 32.04 ± 0.31 | 108 | 66.4 | 61.9 | 67.0 | 72.1 |
| 4.3 | 0.13 | 32.26 ± 0.26 | 0.134 | 32.56 ± 0.12 | 107 | 65.6 | 59.1 | 70.1 | 72.8 |
a
The theoretical molecular mass of L289Ktr monomer is 64.4 kDa.
b
Determined using primusqt from the ATSAS 2.8 software package.
c
Determined using SAXS MoW2.