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. 2022 Apr 11;25(5):104223. doi: 10.1016/j.isci.2022.104223

Figure 4.

Figure 4

Primary human placenta cells can be infected with SARS-CoV-2 ex vivo

(A) qRT-PCR analysis of relative viral N subgenomic RNA expression in primary placental cell clusters infected with SARS-CoV-2 ex vivo (MOI = 1) at 24 hpi and normalized to ACTB levels (mean ± SD; n = 12 from four independent experiments; Student’s t test, ∗∗∗∗p < 0.0001).

(B) Three-dimensional reconstruction of confocal imaging of primary placental cell clusters infected with SARS-CoV-2 ex vivo (MOI = 1) at 24 hpi, stained for trophoblast marker KRT7 (green), SARS-N (red), endothelial marker CD31 (grey), and DAPI (blue). Scale bar = 30 μm.

(C) Confocal imaging of primary placental cell clusters infected with MOCK (top rows) or SARS-CoV-2 (MOI = 1, bottom rows) ex vivo at 24 hpi, stained for trophoblast marker KRT7 (green), SARS-N (red), endothelial marker CD31 (grey), and DAPI (blue). Arrows indicate the presence of SARS-N nucleocapsid protein in trophoblast and endothelial cells. Scale bar = 20 μm.

See also Video S1.