Fig. 5.

YBX1 regulates lung cancer cell progression through NF-κB pathway. Results from (A) CCK-8 (at 24, 48, 72 and 96 h after transfection) and (B) EdU assays (at 48 h after transfection) showing the effect of YBX1 knockout (KO) on A549 cell proliferation. (C) Flow cytometry using Annexin V-FITC/PI staining was performed to analyze apoptosis of YBX1 knockout A549 cells (at 24 h after transfection). The effect of YBX1 knockout on A549 (D) migration and (E) invasion of A549 cells was examined by transwell assay (at 24 h after transfection). F EdU assays showing the influences of YBX1 plasmid transfection and NF-κB inhibitor (IMD-0354, 5 μM, 24 h) treatment on cell proliferation in YBX1-KO A549 cell (at 48 h after transfection). G Transwell assays for analysis the influences of YBX1 plasmid transfection and NF-κB inhibitor (IMD-0354, 5 μM, 24 h) treatment on cell migration in YBX1-KO A549 cell (at 36 h after transfection). Data from three independent experiments are shown as the mean ± SD (error bars). *P < 0.05, **P < 0.01, ***P < 0.001 (Student’s t-test). NC: negative control; WT: wild type