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. 2022 Apr 11;13:45. doi: 10.1186/s40104-022-00687-2

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Immunostaining and quantification of DBA⁺ or UCHL1⁺ cells in porcine testis sections. A Left: DBA immunostaining of porcine testis sections at each age. Bar = 10 μm. Right: the numbers of DBA⁺ cells (upper) and the ratios of DBA⁺ to SOX9⁺ cells (lower) per cross-section of seminiferous cords/tubules in porcine testes at each age. B Left: UCHL1 immunostaining of porcine testis sections at each age. Arrows and arrowheads point to UCHL1^high and UCHL1^low cells, respectively. Bar = 10 μm. Right: the numbers of UCHL1⁺ cells (upper) and the ratios of UCHL1⁺ to SOX9⁺ cells (lower) per cross-section of seminiferous cords/tubules in porcine testes at each age. Data are presented as the mean ± SEM of three littermates, with 50 round cord/tubule cross-sections analyzed per individual. Different letters indicate significant differences between groups (P < 0.05)