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. 2022 Apr 11;20:26. doi: 10.1186/s12969-022-00680-z

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Influence of stimuli on suppressive function of regulatory T cells on peripheral blood mononuclear cells. Isolated regulatory T cells (Treg) and autologous Peripheral blood mononuclear cells (PBMC) were co-cultured in different stimuli. The suppressive function of Treg on PBMC as inhibition in % was calculated as 1-(proliferation of PBMC in 1:1 Co-Culture with isolated Treg × 100) ÷ (proliferation of PBMC in 1:0 Culture) using Carboxyfluorescein-succinimidyl-ester-(CFSE)-assay, assessed by flowcytometry after 5 days of culture. Values are shown in percentage (median and interquartile ranges). As stimuli were used: unstimulated = none; anti-CD3/CD28 as positive control; Th17 = anti-CD3/CD28 + IL-1β + IL-6 + IL-23 + TGFβ; anti-IL17A = anti-CD3/CD28 + laboratory IL-17A-antibody; Secukinumab = anti-CD3/28 + Secukinumab. N = 7 for each stimulus in healthy controls (HC), except for anti-CD3/anti-CD28 (n = 6) and n = 5 for each stimulus in Juvenile Idiopathic Arthritis (JIA). For stimuli comparison all stimuli were compared with anti-CD3/anti-CD28, which served as positive control. Groups as well as stimuli were compared for differences with Mann–Whitney-U-test. Mitigated inhibitory function was seen in Th17 environment in both groups. Difference between positive control and Th17-inducing cytokine environment in HC was significant (p = 0.005, marked with *) after Bonferroni correction was applied. Difference between negative control (= unstimulated) and positive control in HC was statistically significant as well (p = 0.001, not marked)