Skip to main content
. 2022 Apr 10;23:288. doi: 10.1186/s12864-022-08517-5

Table 3.

RT-PCR primers and annealing temperatures for DE circRNAs

CircRNAs Forward 5' → 3' Reverse 5' → 3' product length (bp) Tm/°C
circ_0018936 AGTCCCTGAGTTACGCCTT TGGCCTTACCGATGATGACA 253 55
circ_0003667 TCCTTCTCTGGGGTTCGAAA TTGCATAAAACTCCTCCCGCTA 136 57
circ_0006661 ACAGGAGCCGATATTTAGAGACC TGCTGCCATAGCGACTTGCC 173 58
circ_0020271 TAATCCGCTGAAGATCCCT CTCATCACACTGTACCCAT 205 54
circ_0018320 CTCTAGAAGCATTGGAGCAC GCAGATGAAGAAAATACGCTCA 222 55
β-actin TCCGTGACATCAAGGAGAAGC CCGTGTTGGCGTAGAGGT 267 52–65