Fig. 4. Hyposmia and anxiety-like behavior in A53T BAC-SNCA Tg mice injected with α-Syn PFFs.
(A,B) Olfactory preference test conducted at 2MPI. (A) Sniffing time on water vs. female urine. WT-PBS (n = 13), Tg-PBS (n = 13), and Tg-PFF (n = 14). Left panel: a two-tailed paired test was performed for each group; *p < 0.05, **p < 0.01, n.s., not significant. Right panel: time difference between water and female urine. One-way ANOVA with Tukey’s post hoc test was performed; *p < 0.05. (B) Sniffing time on water vs. peanut butter. WT-PBS (n = 19), Tg-PBS (n = 19), and Tg-PFF (n = 20). Left panel: a two-tailed paired test was performed for each group; *p < 0.05, n.s., not significant. Right panel: time difference between water and peanut butter. One-way ANOVA with Tukey’s post hoc test was performed; *p < 0.05. (C–E) Light/dark transition test conducted at 7MPI. (C) Time spent in dark and light boxes, respectively. One-way ANOVA was performed within each box; n.s., not significant. (D) Latency to first entry into the light box. A Kruskal-Wallis test with Dunn’s post hoc test was performed; *p < 0.05. (E) Number of transitions between light and dark boxes. One-way ANOVA with Tukey’s post hoc test was performed; *p < 0.05. (F,G) Open field test conducted at 7MPI. (F) Left panel: moving distance plotted every 5 min. Two-way repeated measures (RM) ANOVA was performed; effect of group, F2,37 = 3.24, p = 0.050. One-way ANOVA with Tukey’s post hoc test was performed within each time frame; $ $p < 0.01, WT-PBS vs. Tg-PFF: ##p < 0.01, Tg-PBS vs. Tg-PFF. Middle and right panels: moving distance during the first 10 min and 60 min, respectively. One-way ANOVA with Tukey’s post hoc test was performed; **p < 0.01, n.s., not significant. (G) Center time during the first 10 min. A Kruskal-Wallis test with Dunn’s post hoc test was performed; *p < 0.05. WT-PBS (n = 13), Tg-PBS (n = 13), and Tg-PFF (n = 14). Data are the mean ± SEM.