Table 2.
Canine Influenza A virus subtype H3N8 (H3N8 CIV) agglutination of avian and mammalian red blood cells (RBCs) under different incubation conditions.
| H3N8 CIV HAU‡ |
|||||
|---|---|---|---|---|---|
| 23°C × 30 min |
4°C × 60 min |
||||
| RBC source* | RBC SA† | PBS | PBS/BSA | PBS | PBS/BSA |
| Chicken | 2,3/2,6 Neu5Ac | 32 | 16 | 32 | 16 |
| Turkey | 2,3/2,6 Neu5Ac | 16 | 16 | 16 | 16 |
| Goose | 2,3/2,6 Neu5Ac | 8 | 8 | 8 | 8 |
| Dog | 2,3/2,6 Neu5Ac | 8 | 16 | 16 | 16 |
| Horse | 2,3 Neu5Gc | 2 | – | 2 | – |
| Sheep | 2,3 Neu5Gc | 2 | 1 | 2 | 2 |
| Cow | 2,3 Neu5Gc | – | – | 1 | 1 |
*
Species source of RBCs used in the hemagglutination assays. Avian RBC suspensions were 0.5%, and mammalian RBC suspensions were 1.0% in either phosphate buffered saline (PBS) or PBS/bovine serum albumin (BSA).
†
Predominant sialic acid (SA) linkages (2,3 and/or 2,6) and species (Neu5Ac = N-acetylneuraminic acid; Neu5Gc = N-glycolylneuraminic acid) on RBCs.12,16,21,35
‡
Hemagglutinating unit (HAU) = reciprocal of the last dilution of H3N8 CIV that agglutinated RBCs. Negative indicates that no RBC agglutination occurred by undiluted H3N8 CIV suspension. – = negative.