TABLE 2.
Host E. coli strain | Arabinose in medium | Assay |
E. coli harboring:
|
||
---|---|---|---|---|---|
Vector alone | defA | defB | |||
BL21(DE3) (pLysS) | NAb | Deformylase activity | 1 | 1 | 1,600 |
VECO2068 | 0.2% | MIC (μg/ml) of actinonin | 2 | 2 | 16 |
None | Growth | −c | − | +d |
Enzyme activities were normalized to that of E. coli harboring vector alone.
NA, not applicable.
−, no visible growth.
+, growth.