TABLE 5.
Genotypic characterization of actinonin-resistant S. aureus mutantsa
| S. aureus strain | fmt genotype | MIC (μg/ml) of actinonin in the presence of:
|
|
|---|---|---|---|
| No plasmid | pPV158-1 | ||
| ATCC 25923 | Wild type | 16 | NDb |
| VSAU6011 | A108E | >128 | 4 |
| VSAU6012 | G117V | >128 | ND |
| VSAU6013 | E157Stop | >128 | ND |
| 1-63 | Wild type | 16 | 4 |
| VSAU6014 | Frameshiftc | >128 | 8 |
| RN4220 | Wild type | 16 | 2 |
| VSAU7136 | Deletion | >128 | 4 |
a
Values for uncomplemented strains are repeated from Table 4. For strains that harbored pPV158-1 (which supplies fmt in trans), MICs were determined in the presence of 10 μg of tetracycline per ml.
b
ND, not determined.
c
A frameshift at codon 180 results in translation termination at codon 184.