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. 2022 Apr 6;12(7):937. doi: 10.3390/ani12070937

Figure 2.

Figure 2

BPA induces malformation during X. tropicalis embryonic development. The embryos were exposed to different concentrations of BPA for 12 h; when the BPA was removed, the embryos were at stage 10.5 (early gastrula) ((A) 1–5), and the malformation analysis ended at the later tadpole stage 46 ((A) 6–10). The malformation rates were statistically analyzed at 12 h (stage 10.5) (B) and 72 h (stage 46) (D). The mortality rates were statistically analyzed at 12 h (stage 10.5) (C) and 72 h (stage 46) (E). Different letters above the bars indicate significant differences among groups (a > b > c > d, p < 0.05). n = 300/treatment.